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sp1 flash chromatography purification system  (BIOTAGE)

 
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    Structured Review

    BIOTAGE sp1 flash chromatography purification system
    Sp1 Flash Chromatography Purification System, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sp1+flash+purification+system/sp1+flash+chromatography/pmc10104803__41467_2023_37808_MOESM1_ESM-110-9-8
    Average 90 stars, based on 1 article reviews
    sp1 flash chromatography purification system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Substituted indazole derivatives active as kinase inhibitiors
    Article Snippet: After drying under vacuum, the crude solid was purified by chromatography (Biotage SP1 Flash Purification system) on a silica gel cartridge (Biotage SNAP 100 g) using DCM as eluant A and DCM/MeOH 9:1 as eluant B. Elution with a gradient from A/B 100:0 to 70:30 over 25 CV gave a pink solid that was triturated with EtOAc (50 ml), filtered, washed with EtOAc and dried affording 3.01 g (yield: 61%) of the title compound as a whitish solid.

    Article Title: Substituted indazole derivatives active as kinase inhibitiors
    Article Snippet: The crude residue was purified by flash chromatography (Biotage SP1 Flash Purification system) on a silica gel cartridge (Varian SF40-120 g) using n-hexane as eluant A and EtOAc as eluant B. Elution with a gradient from NB 75:25 to 70:30 over 2 CV then from 70:30 to 0:100 over 2 CV then 100% of B afforded 5.9 g (yield: 84%) of the title compound as a yellow oil (yield: 93%).

    Column Chromatography:

    Article Title: Dihydroxybenzamide compound having HSP90 inhibitory activity or pharmaceutically acceptable salt thereof, and medical use thereof
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (silica gel F254 TLC plates, E, Merck) and UV light was visualized by staining with iodine gas. .. Column chromatography can be performed on medium pressure on silica (Merck Silica gel 40-63 μm) or in Biotage SP1 flash purification system (Biotage SP1) using prepacked silica gel cartridges (Biotage). .. NMR analysis was carried out using ARX-300 (300 MHz or more) manufactured by Bruker.

    Article Title: Rational Design and Synthesis of HSF1-PROTACs for Anticancer Drug Development
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (E, Merck) with visualization under UV light or by staining using iodine. .. Column chromatography and medium-pressure liquid chromatography (MPLC) was conducted on silica (Merck Silica Gel 40–63 m) or performed by using a Biotage SP1 flash purification system with prepacked silica gel cartridges (Biotage, Uppsala, Sweden). .. NMR analyses were carried out using a JNM-ECZ500R (500 MHz) manufactured by Jeol resonance.

    Article Title: Selective targeting of cancer cells using a hydrogen peroxide-activated Hsp90 inhibitor.
    Article Snippet: Heat shock protein 90 (Hsp90) plays an important role in cancer cell proliferation, survival, and migration by regulating the maturation and stabilization of numerous oncoproteins.. Despite significant efforts in developing Hsp90 inhibitors, none of these have been approved for clinical use, mostly due to toxicity, such as liver, cardiac, and retinal toxicity.. To avoid undesirable toxicity, we herein report a hydrogen peroxide-activated Hsp90 inhibitor, Boro-BZide (3), which is capable of selectively targeting cancer cells over normal cells.

    Article Title: Benzo-heterocyclic compound and composition for preventing or treating cancer disease, containing same as active ingredient
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (E, Merck) with visualization under UV light or by staining using iodine gas. .. Column chromatography was conducted under medium pressure on silica (Merck Silica Gel 40-63m) or performed by using a Biotage SP1 flash purification system with a prepacked silica gel cartridge (Biotage). .. NMR analysis was performed using JNM-ECZ500R (500 MHZ) manufactured by Jeol resonance.

    Article Title: Reusable Magnetite Nanoparticle (Fe 3 O 4 NP) Catalyst for Selective Oxidation of Alcohols under Microwave Irradiation
    Article Snippet: Commercially available NPs were purchased from Sigma-Aldrich and Thermo Fisher: γ-Fe 2 O 3 [nanopowder, <50 nm particle size (BET), Sigma-Aldrich], FeO (powder, −10 mesh, ≥99.6% trace metals basis, Sigma-Aldrich), and Fe 3 O 4 [nanopowder, 50–100 nm particle size (SEM), 97% trace metal basis, Sigma-Aldrich]. .. Flash column chromatography was performed on Wakogel 60N, 38–100 μm, as described by Still et al. or on a Biotage SP1 Flash Purification System with prepacked silica cartridges. .. 1 H and 13 C NMR spectra were recorded on a JEOL ECS-400NR NMR spectrometer (391.8 and 98.5 MHz, respectively).

    Purification:

    Article Title: Dihydroxybenzamide compound having HSP90 inhibitory activity or pharmaceutically acceptable salt thereof, and medical use thereof
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (silica gel F254 TLC plates, E, Merck) and UV light was visualized by staining with iodine gas. .. Column chromatography can be performed on medium pressure on silica (Merck Silica gel 40-63 μm) or in Biotage SP1 flash purification system (Biotage SP1) using prepacked silica gel cartridges (Biotage). .. NMR analysis was carried out using ARX-300 (300 MHz or more) manufactured by Bruker.

    Article Title: Rational Design and Synthesis of HSF1-PROTACs for Anticancer Drug Development
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (E, Merck) with visualization under UV light or by staining using iodine. .. Column chromatography and medium-pressure liquid chromatography (MPLC) was conducted on silica (Merck Silica Gel 40–63 m) or performed by using a Biotage SP1 flash purification system with prepacked silica gel cartridges (Biotage, Uppsala, Sweden). .. NMR analyses were carried out using a JNM-ECZ500R (500 MHz) manufactured by Jeol resonance.

    Article Title: Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria
    Article Snippet: HPLC-UV-HRMS analysis was conducted on an UltiMate 3000 system (Thermo Fisher) coupled to an Impact II qTof mass spectrometer (Bruker) with an ACQUITY UPLC BEH C18 column (130 Å, 2.1 mm × 100 mm, 1.7-μm particle size, Waters) at a flow rate of 0.4 ml min −1 (5–95% acetonitrile/water with 0.1% formic acid, vol/vol, 16 min, UV detection wavelength 190–800 nm). .. Flash purification was performed on a Biotage SP1 flash purification system (Biotage) by a C 18 main column (Interchim, PF50C18HP-F0080, 120 g) with a self-packed pre-column (Interchim, PF-DLE-F0012, Puriflash dry-load empty F0012 Flash column) coupled with a UV detector. .. HPLC purification was performed on preparative and semipreparative Agilent 1260 systems coupled to a diode array detector (DAD) and a single quadrupole detector with a C18 ZORBAX Eclipse XDB column (9.4 mm × 250 mm, 5 μm, 3 ml min −1 ; 21.2 mm × 250 mm, 5 μm, 20 ml min −1 ; 50 mm × 250 mm, 10 μm, 40 ml min −1 ).

    Article Title: Selective targeting of cancer cells using a hydrogen peroxide-activated Hsp90 inhibitor.
    Article Snippet: Heat shock protein 90 (Hsp90) plays an important role in cancer cell proliferation, survival, and migration by regulating the maturation and stabilization of numerous oncoproteins.. Despite significant efforts in developing Hsp90 inhibitors, none of these have been approved for clinical use, mostly due to toxicity, such as liver, cardiac, and retinal toxicity.. To avoid undesirable toxicity, we herein report a hydrogen peroxide-activated Hsp90 inhibitor, Boro-BZide (3), which is capable of selectively targeting cancer cells over normal cells.

    Article Title: Benzo-heterocyclic compound and composition for preventing or treating cancer disease, containing same as active ingredient
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (E, Merck) with visualization under UV light or by staining using iodine gas. .. Column chromatography was conducted under medium pressure on silica (Merck Silica Gel 40-63m) or performed by using a Biotage SP1 flash purification system with a prepacked silica gel cartridge (Biotage). .. NMR analysis was performed using JNM-ECZ500R (500 MHZ) manufactured by Jeol resonance.

    Article Title: Reusable Magnetite Nanoparticle (Fe 3 O 4 NP) Catalyst for Selective Oxidation of Alcohols under Microwave Irradiation
    Article Snippet: Commercially available NPs were purchased from Sigma-Aldrich and Thermo Fisher: γ-Fe 2 O 3 [nanopowder, <50 nm particle size (BET), Sigma-Aldrich], FeO (powder, −10 mesh, ≥99.6% trace metals basis, Sigma-Aldrich), and Fe 3 O 4 [nanopowder, 50–100 nm particle size (SEM), 97% trace metal basis, Sigma-Aldrich]. .. Flash column chromatography was performed on Wakogel 60N, 38–100 μm, as described by Still et al. or on a Biotage SP1 Flash Purification System with prepacked silica cartridges. .. 1 H and 13 C NMR spectra were recorded on a JEOL ECS-400NR NMR spectrometer (391.8 and 98.5 MHz, respectively).

    Liquid Chromatography:

    Article Title: Rational Design and Synthesis of HSF1-PROTACs for Anticancer Drug Development
    Article Snippet: Analytical thin layer chromatography was performed on precoated silica gel F254 TLC plates (E, Merck) with visualization under UV light or by staining using iodine. .. Column chromatography and medium-pressure liquid chromatography (MPLC) was conducted on silica (Merck Silica Gel 40–63 m) or performed by using a Biotage SP1 flash purification system with prepacked silica gel cartridges (Biotage, Uppsala, Sweden). .. NMR analyses were carried out using a JNM-ECZ500R (500 MHz) manufactured by Jeol resonance.



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